Evidence for convergent nucleotide evolution and high allelic turnover rates at the complementary sex determiner (csd) gene of western and Asian honey bees

Our understanding of the impact of recombination, mutation, genetic drift and selection on the evolution of a single gene is still limited. Here we investigate the impact of all of these evolutionary forces at the complementary sex determiner (csd) gene which evolves under a balancing mode of selection. Females are heterozygous at the csd gene and males are hemizygous; diploid males are lethal and occur when csd is homozygous. Rare alleles thus have a selective advantage, are seldom lost by the effect of genetic drift and are maintained over extended periods of time when compared to neutral polymorphisms. Here, we report on the analysis of 17, 19 and 15 csd alleles of Apis cerana, Apis dorsata and Apis mellifera honey bees respectively. We observed great heterogeneity of synonymous (pi S) and nonsynonymous (pi N) polymorphisms across the gene, with a consistent peak in exon 6 and 7. We propose that exons 6 and 7 encode the potential specifying domain (csd-PSD) which has accumulated elevated nucleotide polymorphisms over time by balancing selection. We observed no direct evidence that balancing selection favors the accumulation of nonsynonymous changes at csd-PSD (pi N/pi S ratios are all < 1, ranging from 0.6 to 0.95). We observed an excess of shared nonsynonymous changes, which suggests that strong evolutionary constraints are operating at csd-PSD resulting in the independent accumulation of the same nonsynonymous changes in different alleles across species (convergent evolution). Analysis of a csd-PSD genealogy revealed relatively short average coalescence times (~6 million years), low average synonymous nucleotide diversity (pi S < 0.09) and a lack of trans-specific alleles which substantially contrasts with previously analyzed loci under strong balancing selection. We excluded the possibility of a burst of diversification after population bottlenecking and intragenic recombination as explanatory factors, leaving high turn-over rates as the explanation for this observation. By comparing observed allele richness and average coalescence times with a simplified model of csd-coalescence, we found that small long term population sizes (i.e. Ne <104), but not high mutation rates, can explain short maintenance times, implicating a strong impact of genetic drift on the molecular evolution of highly social honey bees

PHID-Coleo - a database identification tool for wood-boring beetles in plant health interceptions: Poster

Recent examples for the introduction of wood-breeding beetles in Europe include the asian longhorn beetles Anoplophora spp. and Aromia bungii (red-necked longhorn beetle). These and other woodboring beetle species pose a high risk of economic damage to trees and wood products. Smaller beetles like the powderpost beetles from the families Bostrichidae and Lyctidae also have the potential for causing considerable damage. These are often not identified adequately during inspections of wood packaging materials, making it impossible to assess their risk for becoming invasive. This project will aim at closing that gap. Our project PHID-Coleo (= Plant Health Identification of Coleoptera) has the objective to develop new diagnostic tools for the identification of potentially invasive and economically important beetles that can be found in wood packaging materials. The identification methods include classical identification keys based on morphological characters as well as molecular methods based on DNA analysis by PCR (barcoding). The methods for species identification will be supplemented by molecular analyses of introduced populations to clarify within species variations. Such methods will make it possible to determine the taxonomic relationship of samples from different areas and to draw conclusions about the introduction pathways, resulting in more efficient monitoring of the invasive species and preventing their spread. PHID-Coleo will build a freely accessible database of relevant species which are potentially invasive.Recent examples for the introduction of wood-breeding beetles in Europe include the asian longhorn beetles Anoplophora spp. and Aromia bungii (red-necked longhorn beetle). These and other woodboring beetle species pose a high risk of economic damage to trees and wood products. Smaller beetles like the powderpost beetles from the families Bostrichidae and Lyctidae also have the potential for causing considerable damage. These are often not identified adequately during inspections of wood packaging materials, making it impossible to assess their risk for becoming invasive. This project will aim at closing that gap. Our project PHID-Coleo (= Plant Health Identification of Coleoptera) has the objective to develop new diagnostic tools for the identification of potentially invasive and economically important beetles that can be found in wood packaging materials. The identification methods include classical identification keys based on morphological characters as well as molecular methods based on DNA analysis by PCR (barcoding). The methods for species identification will be supplemented by molecular analyses of introduced populations to clarify within species variations. Such methods will make it possible to determine the taxonomic relationship of samples from different areas and to draw conclusions about the introduction pathways, resulting in more efficient monitoring of the invasive species and preventing their spread. PHID-Coleo will build a freely accessible database of relevant species which are potentially invasive

Statistical Description of Acoustic Turbulence

We develop expressions for the nonlinear wave damping and frequency correction of a field of random, spatially homogeneous, acoustic waves. The implications for the nature of the equilibrium spectral energy distribution are discussedComment: PRE, Submitted. REVTeX, 16 pages, 3 figures (not included) PS Source of the paper with figures avalable at http://lvov.weizmann.ac.il/onlinelist.htm

Arctic climate change: observed and modelled temperature and sea-ice variability

Changes apparent in the arctic climate system in recent years require evaluation in a century-scale perspective in order to assess the Arctic's response to increasing anthropogenic greenhouse-gas forcing. Here, a new set of century- and multidecadal-scale observational data of surface air temperature (SAT) and sea ice is used in combination with ECHAM4 and HadCM3 coupled atmosphere-ice-ocean global model simulations in order to better determine and understand arctic climate variability. We show that two pronounced twentieth-century warming events, both amplified in the Arctic, were linked to sea-ice variability. SAT observations and model simulations indicate that the nature of the arctic warming in the last two decades is distinct from the early twentieth-century warm period. It is suggested strongly that the earlier warming was natural internal climate-system variability, whereas the recent SAT changes are a response to anthropogenic forcing. The area of arctic sea ice is furthermore observed to have decreased similar to8 x 10(5) km(2) (7.4%) in the past quarter century, with record-low summer ice coverage in September 2002. A set of model predictions is used to quantify changes in the ice cover through the twenty-first century, with greater reductions expected in summer than winter. In summer, a predominantly sea-ice-free Arctic is predicted for the end of this century

Sex Determination in Honeybees: Two Separate Mechanisms Induce and Maintain the Female Pathway

Sex determination in honeybees is realized by the csd and the fem gene that establish and maintain, throughout development, sexual fates via the control of alternative splicing

Estimating the Decadal Predictability of a Coupled AOGCM.

On seasonal time scales, ENSO prediction has become feasible in an operational framework in recent years. On decadal to multidecadal time scales, the variability of the oceanic circulation is assumed to provide a potential for climate prediction. To investigate the decadal predictability of the coupled atmosphere–ocean general circulation model (AOGCM) European Centre-Hamburg model version 5/Max Planck Institute Ocean Model (ECHAM5/MPI-OM), a 500-yr-long control integration and “perfect model” predictability experiments are analyzed. The results show that the sea surface temperatures (SSTs) of the North Atlantic, Nordic Seas, and Southern Ocean exhibit predictability on multidecadal time scales. Over the ocean, the predictability of surface air temperature (SAT) is very similar to that of SST. Over land, there is little evidence of decadal predictability of SAT except for some small maritime-influenced regions of Europe. The AOGCM produces predictable signals in lower-tropospheric temperature and precipitation over the North Atlantic, but not in sea level pressure

Beyond the Fokker-Planck equation: Pathwise control of noisy bistable systems

We introduce a new method, allowing to describe slowly time-dependent Langevin equations through the behaviour of individual paths. This approach yields considerably more information than the computation of the probability density. The main idea is to show that for sufficiently small noise intensity and slow time dependence, the vast majority of paths remain in small space-time sets, typically in the neighbourhood of potential wells. The size of these sets often has a power-law dependence on the small parameters, with universal exponents. The overall probability of exceptional paths is exponentially small, with an exponent also showing power-law behaviour. The results cover time spans up to the maximal Kramers time of the system. We apply our method to three phenomena characteristic for bistable systems: stochastic resonance, dynamical hysteresis and bifurcation delay, where it yields precise bounds on transition probabilities, and the distribution of hysteresis areas and first-exit times. We also discuss the effect of coloured noise.Comment: 37 pages, 11 figure

Global population genetic structure and demographic trajectories of the black soldier fly, Hermetia illucens

Background The black soldier fly (Hermetia illucens) is the most promising insect candidate for nutrient-recycling through bioconversion of organic waste into biomass, thereby improving sustainability of protein supplies for animal feed and facilitating transition to a circular economy. Contrary to conventional livestock, genetic resources of farmed insects remain poorly characterised. We present the first comprehensive population genetic characterisation of H. illucens. Based on 15 novel microsatellite markers, we genotyped and analysed 2862 individuals from 150 wild and captive populations originating from 57 countries on seven subcontinents. Results We identified 16 well-distinguished genetic clusters indicating substantial global population structure. The data revealed genetic hotspots in central South America and successive northwards range expansions within the indigenous ranges of the Americas. Colonisations and naturalisations of largely unique genetic profiles occurred on all non-native continents, either preceded by demographically independent founder events from various single sources or involving admixture scenarios. A decisive primarily admixed Polynesian bridgehead population serially colonised the entire Australasian region and its secondarily admixed descendants successively mediated invasions into Africa and Europe. Conversely, captive populations from several continents traced back to a single North American origin and exhibit considerably reduced genetic diversity, although some farmed strains carry distinct genetic signatures. We highlight genetic footprints characteristic of progressing domestication due to increasing socio-economic importance of H. illucens, and ongoing introgression between domesticated strains globally traded for large-scale farming and wild populations in some regions. Conclusions We document the dynamic population genetic history of a cosmopolitan dipteran of South American origin shaped by striking geographic patterns. These reflect both ancient dispersal routes, and stochastic and heterogeneous anthropogenic introductions during the last century leading to pronounced diversification of worldwide structure of H. illucens. Upon the recent advent of its agronomic commercialisation, however, current human-mediated translocations of the black soldier fly largely involve genetically highly uniform domesticated strains, which meanwhile threaten the genetic integrity of differentiated unique local resources through introgression. Our in-depth reconstruction of the contemporary and historical demographic trajectories of H. illucens emphasises benchmarking potential for applied future research on this emerging model of the prospering insect-livestock sector.Peer reviewe

An ocean model's response to North Atlantic Oscillation like wind forcing

The response of the Atlantic Ocean to North Atlantic Oscillation (NAO)-like wind forcing was investigated using an ocean-only general circulation model coupled to an atmospheric boundary layer model. A series of idealized experiments was performed to investigate the interannual to multi-decadal frequency response of the ocean to a winter wind anomaly pattern. Overall, the strength of the SST response increased slightly with longer forcing periods. In the subpolar gyre, however, the model showed a broad response maximum in the decadal band (12-16 years)

The genomes of two key bumblebee species with primitive eusocial organization

Background: The shift from solitary to social behavior is one of the major evolutionary transitions. Primitively eusocial bumblebees are uniquely placed to illuminate the evolution of highly eusocial insect societies. Bumblebees are also invaluable natural and agricultural pollinators, and there is widespread concern over recent population declines in some species. High-quality genomic data will inform key aspects of bumblebee biology, including susceptibility to implicated population viability threats. Results: We report the high quality draft genome sequences of Bombus terrestris and Bombus impatiens, two ecologically dominant bumblebees and widely utilized study species. Comparing these new genomes to those of the highly eusocial honeybee Apis mellifera and other Hymenoptera, we identify deeply conserved similarities, as well as novelties key to the biology of these organisms. Some honeybee genome features thought to underpin advanced eusociality are also present in bumblebees, indicating an earlier evolution in the bee lineage. Xenobiotic detoxification and immune genes are similarly depauperate in bumblebees and honeybees, and multiple categories of genes linked to social organization, including development and behavior, show high conservation. Key differences identified include a bias in bumblebee chemoreception towards gustation from olfaction, and striking differences in microRNAs, potentially responsible for gene regulation underlying social and other traits. Conclusions: These two bumblebee genomes provide a foundation for post-genomic research on these key pollinators and insect societies. Overall, gene repertoires suggest that the route to advanced eusociality in bees was mediated by many small changes in many genes and processes, and not by notable expansion or depauperation